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| Brilhante,Andreia Fernandes; Dorval,Maria Elizabeth Cavalheiros; Galati,Eunice Aparecida Bianchi; Castro,Vivianne de Oliveira Landgraf de; Rocca,Maria Elizabeth Ghizzi; Facco,Gilberto Gonçalves; Souza,Celeste da Silva Freitas de; Nunes,Vânia Lúcia Brandão. |
| ABSTRACT: The study aimed to investigate Leishmania infection in a population of dogs (92 animals) in a fishing area of Bonito Municipality, and evaluate comparatively the serological methods used, immunoenzymatic assay (ELISA), indirect immunofluorescence antibody test (IFAT), and rapid Dual Path-Platform test (DPP®). Blood and aspirate of bone marrow samples were used and parasitological investigation was also performed, such as parasite isolation in Neal, Novy, Nicolle (NNN) medium culture, Woo technique, Giemsa stained smears and specific identification by polymerase chain reaction (PCR). IFAT revealed 56/92 seropositive, the ELISA 8/92 and the DPP®41/92. Regarding the agreement between the serological tests by the Kappa index, there was a slight... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Domestic dogs; Serodiagnosis; Ecotourism. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300501 |
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| Brilhante,Andreia Fernandes; Nunes,Vânia Lúcia Brandão; Kohatsu,Kleber Augusto; Galati,Eunice Aparecida Bianchi; Rocca,Maria Elizabeth Ghizzi; Ishikawa,Edna Aoba Yassui. |
| AbstractBackground Bonito municipality, known as an area of ecoturism, in Mato Grosso do Sul state, Brazil, is also a focus of visceral and cutaneous leishmaniases, with cases registered in both human and canine populations. This study sought to investigate natural infection by flagellate forms of Leishmania in phlebotomines of the urban area of Bonito.Findings Sand flies were collected fortnightly from October 2005 to July 2006 with modified automatic light traps installed in peridomiciles and animal shelters in the center and on the outskirts of the city. The females were dissected and their guts observed under an optical microscope. A total of 1977 specimens were captured, Lutzomyia longipalpis (88.4 %) and Bichromomyia flaviscutelata (3.0 %) being the... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Bichromomyia flaviscutellata; Lutzomyia longipalpis; Natural infection; Leishmania (Leishmania) amazonensis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100502 |
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